Title:
迅速乾燥アッセイ法の形で核酸を検出する方法
Document Type and Number:
Japanese Patent JP2005507674
Kind Code:
A
Abstract:
The invention relates to the field of diagnostics of nucleic acids, especially to a highly sensitive method for detecting, differentiating and characterizing nucleic acids in the form of a dry rapid test. The inventive dry rapid test contains a chromatographic material that comprises a sample reception zone, a separation zone including a binding area, in which one or more sequence-specific nucleic acid probes are immobilized, and a zone which absorbs a liquid down-stream of the separation zone including a binding area. According to the inventive method, the sequence-specific nucleic acid probes are immobilized via a polymer linker. The method comprises the following steps: i) denaturing, in the case of double-stranded nucleic acids, the nucleic acid to be detected and then neutralizing it, ii) applying the nucleic acid to be detected to the sample reception zone in a run buffer which contains mildly denaturing agents, iii) the nucleic acid moving from the sample reception zone in direction of the liquid-absorbing zone, (iv) contacting the nucleic add to be detected in the binding area of the separation path with the sequence-specific nucleic acid probe and hybridizing it with the sequence-specific nucleic acid probe, v) detecting the nucleic acid or the hybridization of the nucleic acid with the sequence-specific nucleic acid probe via a label that is attached to the nucleic acid to be detected or via the detection of a label of the nucleic acid double strand. The invention further relates to a device for carrying out the method according to the invention.
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Inventors:
Weichenegher Michael
Application Number:
JP2003541591A
Publication Date:
March 24, 2005
Filing Date:
November 05, 2002
Export Citation:
Assignee:
HEIN Life Science Game Bha
International Classes:
G01N33/53; B01D15/00; B01J20/281; B01J20/32; C12M1/00; C12M1/34; C12N15/09; C12Q1/04; C12Q1/68; C12Q1/6834; G01N33/543; G01N33/566; G01N33/569; G01N33/58; G01N30/90; (IPC1-7): C12Q1/68; C12M1/00; C12N15/09; G01N33/53; G01N33/543; G01N33/566; G01N33/569; G01N33/58
Attorney, Agent or Firm:
Hatsushi Shimizu
Kazunori Hashimoto
Koichi Niimi
Kazunori Hashimoto
Koichi Niimi