Title:
サンプル系における微量タンパク質の検出方法
Document Type and Number:
Japanese Patent JP7401136
Kind Code:
B2
Abstract:
A method for detecting a trace protein in a sample system, comprising: providing a primary antibody-modified immunomagnetic bead and a secondary antibody-modified nanoparticle for a to-be-detected protein; mixing and incubating the primary antibody-modified immunomagnetic bead and the secondary antibody-modified nanoparticle with a sample system containing the to-be-detected protein, so as to form a complex having a double-antibody sandwich structure; and allowing the complex to pass through a micro-nano pore device in the form of a single particle and trigger an electrical pulse signal, analyzing the electrical pulse signal to obtain a charge state or a volume/mass state of the complex, and calculating, according to the charge state or the volume/mass state, the amount of the to-be-detected protein in the sample system. In the method of the present application, an immunospecifically binding magnetic bead is used, a charge state of a single magnetic bead complex is detected, and an absolute amount of the trace protein is calculated. The method has a detection limit of down to a single protein molecule, can detect a trace protein at an amount below a lower limit of detection of conventional immunological detections, and can be widely used in immunological detection, microorganism detection, and cell separation, among other fields.
Inventors:
Liu Kwae
Xiong Gui
Wang Jia
Xiong Gui
Wang Jia
Application Number:
JP2022538402A
Publication Date:
December 19, 2023
Filing Date:
November 16, 2020
Export Citation:
Assignee:
RESUN (SHENZHEN) TECH CO., LTD.
International Classes:
G01N15/12; G01N1/28; G01N27/00; G01N33/483; G01N33/53
Domestic Patent References:
| JP2018072181A | ||||
| JP2017120257A |
Foreign References:
| EP2311975A1 | ||||
| US20190277840 | ||||
| EP2573554A1 | ||||
| WO2018199179A1 | ||||
| US20180363035 |
Other References:
Nitinun Varongchayakul,Single-molecule protein sensing in a nanopore: a tutorial,Chem Soc Rev.,2018年11月26日,47(23),pp.8512-8524,doi:10.1039/c8cs00106e.
Attorney, Agent or Firm:
Patent Attorney Corporation ATEN
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