Title:
無細胞系でＤＮＡを編集する方法
Document Type and Number:
Japanese Patent JP7025552
Kind Code:
B2
Abstract:
A method for editing DNA in a cell-free system, the method including: (1) a step of introducing a deletion, substitution or addition at a target site of a DNA in a cell-free system, and (2) a step of amplifying, in a cell-free system, the DNA into which a deletion, substitution or addition has been introduced in step (1), wherein the DNA is amplified under temperature conditions that incubate at a temperature within a range from 20°C to 80°C.
Inventors:
Masayuki Suetsugu
Ayako Tawaraki
Takumi Kano
Ayako Tawaraki
Takumi Kano
Application Number:
JP2020534660A
Publication Date:
February 24, 2022
Filing Date:
July 30, 2019
Export Citation:
Assignee:
Orishiro Genomics Co., Ltd.
International Classes:
C12N15/10; C12Q1/6844
Domestic Patent References:
| JP2016077180A |
Foreign References:
| WO2017199991A1 | ||||
| WO2016080424A1 | ||||
| WO2017123921A1 | ||||
| US20070037196 |
Other References:
SU'ETSUGU, Masayuki et al.,Exponential propagation of large circular DNA by reconstitution of a chromosome-replication cycle,Nucleic Acids Res.,2017年,Vol. 45,pp. 11525-11534
LIU, Yunkun et al.,In vitro CRISPR/Cas9 system for efficient targeted DNA editing,mBio,2015年,Vol. 6; e01714-15,pp. 1-8
SANSBURY, Brett M.,CRISPR-Directed In Vitro Gene Editing of Plasmid DNA Catalyzed by Cpf1 (Cas12a) Nuclease and a Mammalian Cell-Free extract,CRISPR J.,2018年04月,Vol. 1,pp. 191-202
LEI, Chao et al.,The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro,Nucleic Acids Res.,2017年,Vol. 45; e74,pp. 1-7
JIANG, Wenjun et al.,Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters,Nat. Commun.,2015年,Vol. 6; 8101,pp. 1-8
LIU, Yunkun et al.,In vitro CRISPR/Cas9 system for efficient targeted DNA editing,mBio,2015年,Vol. 6; e01714-15,pp. 1-8
SANSBURY, Brett M.,CRISPR-Directed In Vitro Gene Editing of Plasmid DNA Catalyzed by Cpf1 (Cas12a) Nuclease and a Mammalian Cell-Free extract,CRISPR J.,2018年04月,Vol. 1,pp. 191-202
LEI, Chao et al.,The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro,Nucleic Acids Res.,2017年,Vol. 45; e74,pp. 1-7
JIANG, Wenjun et al.,Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters,Nat. Commun.,2015年,Vol. 6; 8101,pp. 1-8
Attorney, Agent or Firm:
Shu Oikawa
Makiko Otsuki
Emi Hattori
Junichi Kobayashi
Makiko Otsuki
Emi Hattori
Junichi Kobayashi
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