Title:
How to reduce the level of 1 in a sample, or a plurality of impurities during protein purification
Document Type and Number:
Japanese Patent JP5938295
Kind Code:
B2
Abstract:
A flow-through process for purifying a target molecule from a Protein A eluate comprising the steps of:(i) contacting the eluate recovered from a Protein A chromatography column with activated carbon, (ii) contacting the flow-through sample from step (i) with an anion exchange chromatography media, (iii) contacting the flow-through sample from step (ii) with a cation exchange chromatography media and (iv) obtaining the flow-through sample from step (iii) comprising the target molecule, wherein the eluate flows continuously through steps (i)-(iii) and wherein level of one or more impurities in the flow-through sample after step (iii) is lower than the level in the eluate in step (i). The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product.
Inventors:
Nanin Bian
Christopher Gillespie
Mashiyu Tay Stone
Mikhail Kozlov
Jie Chien
Martyne siwak
Christopher Gillespie
Mashiyu Tay Stone
Mikhail Kozlov
Jie Chien
Martyne siwak
Application Number:
JP2012179197A
Publication Date:
June 22, 2016
Filing Date:
August 13, 2012
Export Citation:
Assignee:
EMD Millipore Corporation
International Classes:
G01N30/88; G01N30/02; G01N30/26; G01N30/96
Domestic Patent References:
| JP2007525412A | ||||
| JP6187631A | ||||
| JP2010279461A |
Foreign References:
| WO2011037522A1 |
Attorney, Agent or Firm:
Kawaguchi International Patent Office
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