ヒトTh1/Th2分化誘導系及びその用途 - National University Corporation Chiba University

Title:

ヒトTh1/Th2分化誘導系及びその用途

Document Type and Number:

Japanese Patent JP4863452

Kind Code:

B2

Abstract:

To provide a new screening system retrieving compounds associated to Ras/MAPK passage or Ca2+/CN passage, or compounds modifying human Th1/Th2 differentiation induction under the condition in which the prescribed passages are acting.

The invention relates to the Th1/Th2 differentiation inducing method for human naive CD4T cell comprising culturing the human naive CD4T cell in a differentiation inducing medium containing IL-2, IL-12 and anti-IL-4 antibody (Th1 differentiation inducing condition), under stimulation by anti-CD3 antibody, or culturing in a differentiation inducing medium containing IL-2, IL-4 and anti-IFN-γ antibody (Th2 differentiation inducing condition) under stimulation by anti-CD3 antibody, and further culturing under culturing condition in which only anti-IL-4 antibody or anti-IFN-γ antibody is removed from each differentiation inducing medium, without stimulation by anti-CD3 antibody.

Inventors:

Toshinori Nakayama
Yamashita Masakatsu
Ohara Osamu
Koga Hisashi

Application Number:

JP2006093086A

Publication Date:

January 25, 2012

Filing Date:

March 30, 2006

Export Citation:

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Assignee:

National University Corporation Chiba University
Kazusa DNA Research Institute

International Classes:

C12N5/0783; C07K16/28; C12N5/07; C12N15/09; C12Q1/02; C12Q1/68; G01N21/78; G01N33/15; G01N33/50; G01N33/577

Domestic Patent References:

JP2004041121A
JP2005535294A
JP2000509604A

Foreign References:

WO2004015079A1

Other References:

J.Immunol.,1998年,Vol.160, No.12,p.5719-5728
J.Immunol.,2001年,Vol.167, No.10,p.5610-5619
J.Exp.Med.,2000年,Vol.191, No.5,p.847-858

Attorney, Agent or Firm:

Masahiro Abe

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