Title:
ヒトグリオーマ抗原及びその調製方法
Document Type and Number:
Japanese Patent JP3749709
Kind Code:
B2
Abstract:
The present invention provides a human glioma antigen or a method for preparing the same, which can be applied to diagnosis and therapy of glioma. A glioma antigen or a glioma antigen gene is prepared by a process comprising the steps of: total RNA is extracted from a glioma cell line and cDNA was synthesized; lambda phage cDNA library, which was constructed by infection of E. coli caused by introducing said cDNA into lambda phage vector, and the sera of glioma patients are made to react; positive clones to which antibodies in the serum reacted are detected with the use of labeled anti-IgG antibodies; screening is repeated several times for the detected positive clones; antigens are isolated from positive clones confirmed to be antibody-reactive; serum screening is conducted by using the isolated antigens, the sera of glioma patients and healthy persons.
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Inventors:
Masahiro Toda
Yutaka Kawakami
Kawase
Yukihiko Iizuka
Yutaka Kawakami
Kawase
Yukihiko Iizuka
Application Number:
JP2002556742A
Publication Date:
March 01, 2006
Filing Date:
November 30, 2001
Export Citation:
Assignee:
Gbs Institute Inc.
International Classes:
C12N15/09; A01K67/027; A61P35/00; C07K14/47; C07K14/82; C07K16/32; C07K19/00; C12N1/21; C12N5/10; C12N15/12; C12Q1/02; C12Q1/68; G01N33/15; G01N33/50; G01N33/53; G01N33/566; G01N33/574; A61K39/00
Other References:
神経免疫研究,2000年12月20日,第13巻,p.44-46
Proceedings of AACR Annual Meeting,Vol.41(2000),p.880
Proc.Natl.Acad.Sci.USA.,Vol.97,No.7(2000),p.3491-3496
Genome Res.,Vol.6,No.9(1996),p.807-828
Genome Res.,Vol.10(1996),p.1617-1630
Proceedings of AACR Annual Meeting,Vol.41(2000),p.880
Proc.Natl.Acad.Sci.USA.,Vol.97,No.7(2000),p.3491-3496
Genome Res.,Vol.6,No.9(1996),p.807-828
Genome Res.,Vol.10(1996),p.1617-1630
Attorney, Agent or Firm:
Masaki Hirota