Title:
哺乳類遺伝子発現のためのヒトハイブリッド宿主細胞
Document Type and Number:
Japanese Patent JP5826311
Kind Code:
B2
Abstract:
Human/human hybrid cells were made via fusion of human embryonic kidney cells (293S) and modified Burkitt's lymphoma cells (2B8). The fusion cells are useful as host cells for the recombinant expression of mammalian genes. The advantages of using these hybrid clones of human kidney- and B-cells, called HKBs, for mammalian gene expression, include (i) the cells are negative for immunoglobulin expression, (ii) the cells grow easily in plasma protein-free medium (with or without the addition of recombinant insulin) as suspension cultures in a shake flask or in a fermenter (iii) the cells are very susceptible for transfection of DNA, and (iv) the cells secrete high levels of heterologous recombinant proteins, such as recombinant monoclonal antibodies, soluble ICAM-1, rIL-4, and rFVIII.
Inventors:
Miyun-Sam Chiyo
Application Number:
JP2014032748A
Publication Date:
December 02, 2015
Filing Date:
February 24, 2014
Export Citation:
Assignee:
BAYER CORPORATION
International Classes:
C12N5/10; C07K2/00; C12N5/02; C12N5/07; C12N5/073; C12N5/16; C12N5/22; C12N5/24; C12N15/01; C12N15/02; C12N15/07; C12N15/08; C12N
Domestic Patent References:
| JP57040411A |
Other References:
Gene,1989年,vol. 81,p. 139-149
Mol. Cell Biol.,1985年,vol. 5, no. 8,p. 2051-2060
Mol. Cell Biol.,1985年,vol. 5, no. 8,p. 2051-2060
Attorney, Agent or Firm:
Patent business corporation Odashima patent office
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