Title:
ｉｎ ｓｉｔｕ分子検出のためのハイブリダイゼーション連鎖反応法
Document Type and Number:
Japanese Patent JP7259182
Kind Code:
B2
Abstract:
The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.
Inventors:
Doherty, Evan Earl.
Church, George M.
Church, George M.
Application Number:
JP2018555974A
Publication Date:
April 18, 2023
Filing Date:
April 25, 2017
Export Citation:
Assignee:
President and Fellows of Harvard College
International Classes:
C12Q1/6813; C12Q1/6844; G01N33/53
Foreign References:
| WO2015118029A1 | ||||
| WO2014182528A2 |
Attorney, Agent or Firm:
Kato Kazuho
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