Title:
HYBRIDOMA CELL SYSTEM AND MONOCLONAL ANTIBODY
Document Type and Number:
Japanese Patent JPS60110288
Kind Code:
A
Abstract:
Monoclonal antibodies that recognize a structure common to human interleukin-2 and to the light lambda chain of human immunoglobulin and lines of hybridoma cells that produce these monoclonal antibodies can be prepared by immunizing animals, especially mice, with human interleukin-2 (TCGF) and fusing the splenocytes obtained from the animals with animal, expecially mouse, myeloma cells to create a hybridoma. The hybridomas are raised as clones and the antibodies obtained from the individual clones tested for their specificity to human interleukin-2 (TCGF). Clones that produce antibodies with a specificity to human interleukin-2 (TCGF) are selected for further raising to prepare the antibody. The antibody is harvested from the culture medium or from the ascitic fluids of the animal, especially the mouse, with the hybridoma.
Inventors:
HANSU HAA ZONNEBOON
RORUFU EMU FUORUNHAAGEN
UDO JIYUBUARERA
RORUFU EMU FUORUNHAAGEN
UDO JIYUBUARERA
Application Number:
JP17009584A
Publication Date:
June 15, 1985
Filing Date:
August 16, 1984
Export Citation:
Assignee:
BIOTEST SERUM INSTITUT GMBH
International Classes:
G01N33/577; A61K39/395; C07K14/435; C07K14/52; C07K14/54; C07K14/55; C07K16/00; C07K16/24; C12N5/10; C12N15/02; C12P21/08; A61K38/00; C12R1/91; (IPC1-7): A61K39/395; C07K15/06; C12N5/00; C12N15/00; C12P21/00; C12R1/91; G01N33/577
Attorney, Agent or Firm:
Kinichi Kitamura
Previous Patent: JPS60110287
Next Patent: MONOCLONAL ANTIBODY HAVING HIGH COMPATIBILITY WITH ZIGOXINE
Next Patent: MONOCLONAL ANTIBODY HAVING HIGH COMPATIBILITY WITH ZIGOXINE