Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
HYDRATED ZIRCONIUM SILICATE COMPOSITION FOR PURIFYING NUCLEIC ACID
Document Type and Number:
Japanese Patent JPH10147592
Kind Code:
A
Abstract:

To obtain the subject zirconium silicate composition for purifying a nucleic acid, capable of more effectively and efficiently purifying the nucleic acid from a sample and used for a solid-phase extraction method, etc., for the nucleic acid by using a hydrated zirconium silicate bondable to the nucleic acid.

An aqueous solution of sodium hydroxide is added to zirconium silicate beads and the resultant suspension is then refluxed for 72hr and cooled to ambient temperature. A 10% sulfuric acid is added to acidify the cooled suspension and the prepared acidic suspension is stirred at ambient temperature for 1hr, filtered and then washed with water three times and then with acetone three times and further dried in an oven at 100°C for 1hr to afford the objective hydrated zirconium silicate composition, having at least 2.0mg/μL density and bonding properties to the nucleic acid, capable of more effectively and efficiently purifying the nucleic acid from a sample and usable for a solid-phase extraction method, etc., for the nucleic acid.


Inventors:
WOODARD DANIEL L
Application Number:
JP27806097A
Publication Date:
June 02, 1998
Filing Date:
September 25, 1997
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
BECTON DICKINSON CO
International Classes:
C07H1/06; C01G25/00; C07H21/00; C12N15/10; (IPC1-7): C07H21/00; C01G25/00; C07H1/06
Attorney, Agent or Firm:
Nao Okuyama (3 others)