Title:
ヒドロラーゼ、それをコードする核酸並びにその製造および使用方法
Document Type and Number:
Japanese Patent JP2007524375
Kind Code:
A
Abstract:
The disclosure provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the disclosure is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the disclosure include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the disclosure can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the disclosure are used to synthesize enantiomerically pure chiral products.
Inventors:
Bornshore Uwe
Weiner David Paul
Hitchman tim
Ryan Jonathan
Wonthakuru Cyril
Weiner David Paul
Hitchman tim
Ryan Jonathan
Wonthakuru Cyril
Application Number:
JP2006509241A
Publication Date:
August 30, 2007
Filing Date:
March 08, 2004
Export Citation:
Assignee:
Diversa Corporation
International Classes:
C12N15/09; A01H1/00; A01H5/00; A01K67/027; A23C7/00; A23C9/20; A23C19/05; A23C19/06; A23D7/00; A23K1/16; A23K20/189; A23L1/00; A23L1/30; A23L29/00; A23L33/17; A23L33/195; A61K6/00; A61K8/37; A61K8/60; A61K8/66; A61K31/7088; A61K31/7105; A61K38/00; A61K38/46; A61K48/00; A61P39/02; A61P43/00; A61Q5/06; A61Q11/00; A61Q19/00; C07H21/00; C07H21/02; C07K16/40; C07K19/00; C10M129/70; C10M129/76; C10M177/00; C11D3/386; C11D7/42; C11D17/06; C11D17/08; C12C1/047; C12C5/00; C12C11/00; C12M1/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/14; C12N9/16; C12N9/18; C12N9/20; C12N9/48; C12N9/50; C12N9/80; C12N11/00; C12N11/02; C12N11/14; C12N11/16; C12N15/10; C12N15/55; C12N15/82; C12P7/52; C12P7/62; C12P7/64; C12P19/34; C12P21/08; C12P41/00; C12Q1/02; C12Q1/04; C12Q1/34; C12Q1/37; C12Q1/44; C12Q1/68; C12S11/00; D06M15/00; D06M16/00; G01N33/573; G06F19/00; A61K
Domestic Patent References:
| JPH0246280A | 1990-02-15 | |||
| JP2001504327A | 2001-04-03 |
Foreign References:
| WO2002020735A2 | 2002-03-14 | |||
| WO2002057411A2 | 2002-07-25 | |||
| US5089395A | 1992-02-18 |
Other References:
JPN6010014925, Ｊ． Ｂｉｏｌ． Ｃｈｅｍ．, 1995, ２７０（４６）, ２７７６６−２７７７２
JPN6010014926, Ｂｉｏｃｈｉｍ． Ｂｉｏｐｈｙｓ． Ａｃｔａ, 2003, １６３１, １９７−２０５
JPN6010014928, Ａｎａｌ． Ｂｉｏｃｈｅｍ．, 1967, ２１, ２７９−２８５
JPN6010014927, Ａｎａｌ． Ｂｉｏｃｈｅｍ．, 2003, ７５, ２５５−２６０
JPN6010014929, Ｃｌｉｎ． Ｃｈｅｍ．, 1975, ２１（１２）, １７８８−１７９０
JPN6010014924, Ａｎｇｅｗ． Ｃｈｅｍ． Ｉｎｔ． Ｅｄ．, 2003, ４２（１２）, １４１８−１４２０
JPN6010014926, Ｂｉｏｃｈｉｍ． Ｂｉｏｐｈｙｓ． Ａｃｔａ, 2003, １６３１, １９７−２０５
JPN6010014928, Ａｎａｌ． Ｂｉｏｃｈｅｍ．, 1967, ２１, ２７９−２８５
JPN6010014927, Ａｎａｌ． Ｂｉｏｃｈｅｍ．, 2003, ７５, ２５５−２６０
JPN6010014929, Ｃｌｉｎ． Ｃｈｅｍ．, 1975, ２１（１２）, １７８８−１７９０
JPN6010014924, Ａｎｇｅｗ． Ｃｈｅｍ． Ｉｎｔ． Ｅｄ．, 2003, ４２（１２）, １４１８−１４２０
Attorney, Agent or Firm:
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Koji Hirayama