Title:
【発明の名称】二重酵素標識化結合パートナーを用いる化学発光検出法
Document Type and Number:
Japanese Patent JP2001504226
Kind Code:
A
Abstract:
Methods of detecting analytes or target species using two enzyme-labeled specific binding partners where the two enzymes function in concert to produce a detectable chemiluminescent signal are disclosed. The methods use a specific binding partner labeled with a hydrolytic enzyme to produce a phenolic enhancer in close proximity to a peroxidase-labeled second specific binding partner. The method is useful to detect and quantitate with improved specificity various biological molecules including antigens and antibodies by the technique of immunoassay, proteins by Western blotting, DNA by Southern blotting, RNA by Northern blotting. The method may also be used to detect DNA mutations and juxtaposed gene segments in chromosomal translocations and particularly to unambiguously identify heterozygous genotypes in a single test.
Inventors:
Akhavan-Tafty, Hashem
Sugioka, Katsuaki
Sugioka Yumiko
Lady, wrecker buoy
Sugioka, Katsuaki
Sugioka Yumiko
Lady, wrecker buoy
Application Number:
JP52259598A
Publication Date:
March 27, 2001
Filing Date:
November 07, 1997
Export Citation:
Assignee:
Lumigen ink
International Classes:
C07D219/02; C07D219/04; C07D219/06; G01N33/535; C09B15/00; C09K11/07; C12Q1/28; C12Q1/34; C12Q1/68; G01N21/78; G01N33/53; G01N33/543; G01N33/58; (IPC1-7): G01N33/535; C12Q1/68; G01N33/543
Attorney, Agent or Firm:
Masatake Shiga (1 person outside)