Title:
【発明の名称】ポリヒドロキシアルカノエートの産生に有用なDNA配列
Document Type and Number:
Japanese Patent JP2000516813
Kind Code:
A
Abstract:
A genomic fragment harboring the gene phaG was cloned by phenotype complementation of Pseudomonas putida KT2440 mutants, defective in the polyhydroxyalkanoic acid (PHA) synthesis via de novo fatty acid biosynthesis but not affected in PHA biosynthesis via fatty acid beta-oxidation. The 885-bp phaG gene encodes a protein of 295 amino acids with a molecular weight of 33.876 Da. The transcriptional induction of phaG could be observed under conditions of PHA synthesis via de novo fatty acid biosynthesis whereas no induction was detected under conditions which favor PHA synthesis via fatty acid degradation by beta-oxidation. The phaG gene is useful for the production of PHAs in bacteria and plants.
Inventors:
Crigel, Niles
Alexander Beutzchel, Alexander
Alexander Beutzchel, Alexander
Application Number:
JP51005798A
Publication Date:
December 19, 2000
Filing Date:
August 13, 1997
Export Citation:
Assignee:
Monsanto Company
International Classes:
A01H5/00; C12N1/14; C12N1/20; C12N1/21; C12N5/10; C12N9/00; C12N9/10; C12N15/09; C12N15/31; C12N15/54; C12N15/82; C12P7/62; (IPC1-7): C12N15/09; A01H5/00; C12N1/14; C12N1/20; C12N1/21; C12N5/10; C12N9/00; C12P7/62
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)