Title:
【発明の名称】改良されたキメラ毒素
Document Type and Number:
Japanese Patent JP3311346
Kind Code:
B2
Abstract:
A chimeric toxin comprising protein fragments joined together by peptide bonds, the chimeric toxin comprising, in sequential order, beginning at the amino terminal end of the chimeric toxin, (a) the enzymatically active Fragment A of diphtheria toxin, (b) a first fragment including the cleavage domain 11 adjacent the Fragment A of diphtheria toxin, (c) a second fragment comprising at least a portion of the hydrophobic transmembrane region of Fragment B of diphtheria toxin, the second fragment having a deletion of at least 50 diphtheria toxin amino acid residues, the deletion being C-terminal to the portion of the transmembrane region, and the second fragment not including domain 12, and (d) a third fragment comprising a portion of a cell-specific polypeptide ligand, the portion including at least a portion of the binding domain of the polypeptide ligand, the portion of the binding domain being effective to cause the chimeric toxin to bind selectively to a predetermined class of cells to be attacked by the enzymatically active Fragment A.
Inventors:
Williams, Diane
John Earl Murphy
John Earl Murphy
Application Number:
JP50604891A
Publication Date:
August 05, 2002
Filing Date:
February 28, 1991
Export Citation:
Assignee:
Boston Medical Center Corporation
International Classes:
A61K9/00; A61K9/06; A61K9/08; A61K38/095; A61K38/22; A61K38/24; A61K38/27; A61K38/28; A61K38/35; A61P35/00; C07H21/04; C07K14/00; C07K14/195; C07K14/34; C07K14/41; C07K14/485; C07K14/50; C07K14/52; C07K14/54; C07K14/55; C07K14/575; A61K38/00; C07K19/00; C12N1/21; C12N15/09; C12N15/31; C12N15/62; C12P21/02; C12R1/19; (IPC1-7): C12N15/09; A61K9/00; A61K9/06; A61K9/08; A61K38/00; C07H21/04; C07K14/34; C07K14/50; C07K19/00; C12N1/21; C12P21/02
Other References:
【文献】米国特許4675382(US,A)
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)