Title:
【発明の名称】微生物のインジゴ産生時のナフタレン・ジオキシゲナーゼ活性の増大
Document Type and Number:
Japanese Patent JP2974407
Kind Code:
B2
Abstract:
This invention relates to a process for the enhancement of naphthalene dioxygenase activity in organisms that have been transformed with DNA encoding the expression of a multiple component naphthalene dioxygenase enzyme. Cells containing the naphthalene dioxygenase enzyme are capable of producing indigo when cultured in the presence of indole. The invention includes procedures to enhance naphthalene dioxygenase activity by transforming a host cell with a DNA sequence that encodes at least two ferredoxin polypeptides for each reductase polypeptide and iron-sulfur polypeptide, by growing transformed organisms in the presence of iron, or by transforming a host cell with a DNA sequence that encodes a ferredoxin analog.
Inventors:
SADAARU KUNEI EMU
MAADOTSUKU DAGURASU SHII
ENZUREE BAATO DEII JUNIA
MAADOTSUKU DAGURASU SHII
ENZUREE BAATO DEII JUNIA
Application Number:
JP51162690A
Publication Date:
November 10, 1999
Filing Date:
July 19, 1990
Export Citation:
Assignee:
AMUJEN
International Classes:
C07K14/79; C12N9/02; C12N15/53; C12P17/16; (IPC1-7): C12N15/09; C12N1/21
Domestic Patent References:
| JP59501972A |
Attorney, Agent or Firm:
Yoshihiro Tsunoda