Title:
【発明の名称】粒子トランスフェクション:細胞へのポリヌクレオチド分子の転移方法
Document Type and Number:
Japanese Patent JPH08504321
Kind Code:
A
Abstract:
A rapid, simple method of directly transfecting a large number of eukaryotic, prokaryotic or plant cells, which retains substantial cell viability is achieved by the present invention. The method includes the steps of contacting with cells adhered to a support, an amount of polynucleotide molecule targeted for transfection into the cells and an amount of particles. A gentle agitation of the cells, polynucleotide molecules and particles permits direct transfection of the polynucleotide molecules into the cells.
Inventors:
Caretings, Armand
Matthews, Catherine, E.
Mills, Gordon, Bee.
Matthews, Catherine, E.
Mills, Gordon, Bee.
Application Number:
JP50947493A
Publication Date:
May 14, 1996
Filing Date:
October 13, 1993
Export Citation:
Assignee:
Cangene Corporation
International Classes:
C12N15/09; C12N15/87; (IPC1-7): C12N15/09
Attorney, Agent or Firm:
Fukuda Hideyuki