Title:
【発明の名称】蛍光体でマーキングしたDNAおよびRNAの光励起法
Document Type and Number:
Japanese Patent JP2003506677
Kind Code:
A
Abstract:
A method for optical excitation of fluorophore-labeled DNA and fluorophore-labeled RNA, particularly of specific localizations of DNA and RNA labeled by fluorescence in situ hybridization (FISH). It is the object of the method to make possible in a simple manner a high-contrast simultaneous excitation of a plurality of FISH fluorophores which have different fluorescence characteristics and are to be detected and displayed three-dimensionally. The excitation and detection of fluorophores at a depth of the biological material greater than 100 micrometers must be ensured. The FISH fluorophores and DNA markers are excited to fluorescence by a multiphoton excitation simultaneously by pulsed and non-pulsed radiation at a single wavelength in the range between 700 nm to 1000 nm, preferably between 760 nm and 820 nm. A total of 20 commercially available FISH fluorophores and DNA markers were tested. Fluorophores which were excited simultaneously by multiphoton excitation were detected in all tested cases.
Inventors:
Canic carsten
Harbaubel Karl Jürgen
Fisher Peter
Lehman Iris
Harbaubel Karl Jürgen
Fisher Peter
Lehman Iris
Application Number:
JP2001514553A
Publication Date:
February 18, 2003
Filing Date:
July 11, 2000
Export Citation:
Assignee:
Carl Zeiss Jena Gezel Shaft Mitt Beschlenktel Haftung
International Classes:
G01N21/64; C12Q1/68; C12Q1/6841; G01N33/48; G01N33/50; G01N33/58; (IPC1-7): G01N21/64; G01N33/48; G01N33/50; G01N33/58
Attorney, Agent or Firm:
Matsuda