Title:
【発明の名称】タンパク質パートナースクリーニング検定法およびその使用
Document Type and Number:
Japanese Patent JPH05507193
Kind Code:
A
Abstract:
A rapid, simple and inexpensive method to screen and classify proteins as partners of heterodimeric proteins is described which utilizes fusion protein constructs containing a DNA binding domain and a dimerization domain from a different protein. According to the method of the invention, heterodimer formation is detected by the ability of the protein partner to displace homodimer formation, and thus reveal a phenotypic change in a bacterial host which was dependent upon maintenance of the homodimer configuration. The method of the invention may be used to identify compounds of interest which inhibit such heterodimer formation, and especially to identify compounds which prevent heterodimer formation and activation of oncogenic transcriptional regulatory proteins.
Inventors:
Robert E. Kingston
Bunker, Christopher Alden
Bunker, Christopher Alden
Application Number:
JP50760391A
Publication Date:
October 21, 1993
Filing Date:
March 26, 1991
Export Citation:
Assignee:
The General Hospital Corporation
International Classes:
C07K14/01; C07K14/82; C12N15/10; C12N15/09; C12N15/12; C12N15/62; C12Q1/02; C12Q1/68; C12Q1/70; G01N33/50; G01N33/68; (IPC1-7): C12N15/62; C12N15/12
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)