【発明の名称】ジゴキシゲニン誘導体、その製法、標識結合体の製法、核酸検出法及びジゴキシゲニン結合体

Japanese Patent JPH0637513

B2

Digoxigenin derivatives of the formula (I) in which n is an integer from 1 to 4 and Z is a carboxyl group or a functional derivative thereof, and digoxigenin conjugates of the formula (II) in which the carrier is an immunogenic carrier material, a nucleic acid or the labelled structural unit of a labelled digoxigenin conjugate for use in immunoassays, y is, on average, a number from 1 to the number of coupling sites available in the carrier, and Y is the group formed by reaction of the carboxyl group Z of the digoxigenin derivatives of the formula (I) with the reacting sites on the carrier material, are described.     The digoxigenin derivatives of the formula (I) are used for preparing labelled conjugates for determining cardiotonic glycosides, especially digoxin, in immunoassays and as marker hapten for detecting nucleic acids. The digoxigenin conjugates of the formula (II) in which the carrier is an immunogen can be employed for preparing antibodies which are used in immunoassays for determining cardiotonic glycosides, and the digoxigenin conjugates of the formula (II) in which the carrier is a labelled structural unit are used as competitive component in immunoassays of this type.

Erasmus Hoover
Klaus Miyuletger
Herbert Huon del Erz
Bruno Twink

JP27879989A

May 18, 1994

October 27, 1989

Click for automatic bibliography generation  

Boehringer Mannheim Geselsyaft Mitt Besilyenktel Haftung

A61K39/00; C07J19/00; C07J41/00; C07J43/00; C07K1/113; C07K14/00; C12Q1/68; C12Q1/6816; G01N33/53; G01N33/532; (IPC1-7): C07J19/00; A61K39/00; C07K15/16; G01N33/53

Toshio Yano (2 outside)

Next Patent: SUPERCONDUCTIVE HIGH FREQUENCY CIRCUIT DEVICE