Title:
【発明の名称】外来タンパク質を分泌することを目的としたBAR1の使用法
Document Type and Number:
Japanese Patent JP2523562
Kind Code:
B2
Abstract:
A method for producing a foreign protein in a host organism whereby the protein is processed through the secretory pathway of the host. Production and processing is achieved by transforming the host organism with a vector comprising at least the signal peptide coding region of the yeast S. cerevisiae BAR1 gene fused to a foreign structural gene. DNA constructs and transformants are also provided containing a region of the BAR1 gene encoding the signal peptide and a KEX2 processing site, and a foreign structural gene. In particular, proinsulin is expressed and secreted from Schizosaccharomyces pombe and Saccharomyces cerevisiae.
Inventors:
MATSUKEI UIUIAN ERU
Application Number:
JP50571386A
Publication Date:
August 14, 1996
Filing Date:
October 20, 1986
Export Citation:
Assignee:
ZAIMOJENETEITSUKUSU INC
International Classes:
C12N15/00; C07K14/575; C07K14/62; C07K19/00; C12N1/14; C12N1/15; C12N1/16; C12N1/18; C12N1/19; C12N9/60; C12N15/09; C12N15/62; C12N15/81; C12P21/02; C12R1/645; C12R1/865; (IPC1-7): C12N15/09; C12N1/15; C12N1/19
Domestic Patent References:
| JP61247384A |
Attorney, Agent or Firm:
Minoru Nakamura (7 outside)