Title:
【発明の名称】G?ンパ?質共役レ?プ?ー活性のス?リーニング方法
Document Type and Number:
Japanese Patent JPH11505718
Kind Code:
A
Abstract:
A method for screening G protein coupled receptors is provided in which G protein coupled receptors that are constitutively active are determined, such as by measuring receptor phosphorylation agonist independent signalling. When a G protein coupled receptor is found to be regulated by constitutive activity, then assay systems may be set up to classify test compounds as agonists, neutral antagonists, or negative antagonists with respect to G protein coupled receptor signalling and phosphorylation. Such determinations and screening are useful for selecting new pharmaceuticals potentially useful in treating disease states mediated by G protein coupled receptors, with applications including treatments in conjunction with narcotic analgesia.
Inventors:
Sadie, Wolfing
Application Number:
JP53582196A
Publication Date:
May 25, 1999
Filing Date:
May 21, 1996
Export Citation:
Assignee:
The Regents of the University of California
International Classes:
G01N33/15; A61K31/47; A61K31/485; A61K31/495; A61K31/496; A61K31/522; A61K38/00; A61K38/08; A61K45/00; A61P25/30; A61P39/02; C07K14/705; C07K14/72; C12Q1/02; C12Q1/48; G01N33/53; G01N33/566; G01N33/94; (IPC1-7): C12Q1/48; A61K38/00; A61K45/00; G01N33/53; G01N33/566
Attorney, Agent or Firm:
Atsushi Nakajima (2 outside)