Title:
【発明の名称】サイクリングプローブ反応によるバンコマイシン耐性腸球菌を検出するための組成物および方法
Document Type and Number:
Japanese Patent JP2002507129
Kind Code:
A
Abstract:
A method for determining the presence of vancomycin antibiotic resistant gene of enterococci in a biological sample, comprising the steps of (a) treating cells contained within the biological sample to expose single stranded-target nucleic acid molecules; (b) reacting the target single-stranded cellular nucleic acids with probe(s) nucleic acid sequence complementary to a portion of the antibiotic vancomycin resistant gene and the probe having a scissile linkage, and with an enzyme molecule, under conditions, which allow the target and probe to hybridize to each other and form a double-stranded, target-probe complex, the enzyme molecule being capable of cleaving the scissile link of the target-probe complex such that one or more fragments of the nucleic acid probe is released from said complex; and (c) determining whether cleaved portions of the nucleic acid probe are produced, and thereby detecting the presence of a vancomycin antibiotic resistant gene.
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Inventors:
Modula Sun, Zola Dee.
Application Number:
JP50600899A
Publication Date:
March 05, 2002
Filing Date:
July 03, 1998
Export Citation:
Assignee:
ID Biomedical Corporation
International Classes:
C12N15/09; C07H21/04; C12P19/34; C12Q1/68; (IPC1-7): C12Q1/68; C12N15/09
Attorney, Agent or Firm:
Takeshi Oshio