Title:
【発明の名称】内皮細胞産物を製造するために脂肪組織を処理する装置及び方法
Document Type and Number:
Japanese Patent JPH07509369
Kind Code:
A
Abstract:
Methods and apparatus for collecting and processing tissue to produce an endothelial cell product having a vessel for rinsing, draining, digesting and isolating tissue. The vessel has a rinsing and digesting chamber for containing tissue during processing. An inlet in the rinsing and digesting chamber allows entry of rinsing solution and tissue from a liposuction device. A waste chamber in fluid communication with the rinsing and digesting chamber preferably connects with a vacuum source. An isolation chamber is separated from the rinsing and digesting chamber by a screen. An ampule in fluid communication with the isolation chamber includes a pair of ports controlled by valve devices to be selectively in fluid communication with the isolation chamber. After processing, the ampule isolates a pellet of endothelial cells and the valve devices permit the pellet to be in fluid communication with the ports. The method includes providing the vessel, introducing tissue to be processed, orienting the vessel to screen the tissue, introducing an enzyme and agitating to digest the tissue, centrifuging the vessel to transfer the cells from the digested tissue, and isolating the cells for retrieval.
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Inventors:
Archas, Paul Gee
Price, Alfred W
Jarrell, Bruce Yee
Stuart Kay, Williams
DiPisa, Joseph A., Junior
Price, Alfred W
Jarrell, Bruce Yee
Stuart Kay, Williams
DiPisa, Joseph A., Junior
Application Number:
JP50547593A
Publication Date:
October 19, 1995
Filing Date:
July 30, 1993
Export Citation:
Assignee:
Thomas Jefferson University
Becton Dickinson and Company
Becton Dickinson and Company
International Classes:
C12M3/00; A61F2/06; A61L27/34; A61L27/38; A61L27/50; C12M3/08; C12N5/00; C12N5/07; C12N5/077; C12S3/00; (IPC1-7): C12M3/00; C12N5/06
Attorney, Agent or Firm:
Kyozo Yuasa (6 people outside)