Title:
【発明の名称】プラスミドDNAによる植物プロトプラストの試験管内形質転換方法
Document Type and Number:
Japanese Patent JPH0630589
Kind Code:
B2
Abstract:
Genetic properties of higher plants are transformed by incubating protoplasts of the plants together with plasmid DNA. The incubation is effected in the presence of polyethylene glycol and calcium ions. Subsequently-during after-incubation-the calcium ion concentration in the incubation medium is gradually increased and at the same time the polyethylene glycol concentration is decreased. The resulting aggregates are separated, grown further separately, and examined for modification of their genetic properties.
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Inventors:
ROBAATO ADORIAN SHIRUPAROORUTO
FURANSHISUKASU ANDORIIZU KURENSU
JOOJI YOZEFU UORUMUZU
FURANSHISUKASU ANDORIIZU KURENSU
JOOJI YOZEFU UORUMUZU
Application Number:
JP1986583A
Publication Date:
April 27, 1994
Filing Date:
February 10, 1983
Export Citation:
Assignee:
REIKUSUYUNIBERUSHITEETO TE FURONINGEN
ROBAATO ADORIAN SHIRUPAROORUTO
ROBAATO ADORIAN SHIRUPAROORUTO
International Classes:
A01H1/00; A01H1/02; A01H5/00; C12N5/00; C12P13/04; C12N5/10; C12N9/00; C12N15/00; C12N15/09; C12N15/63; C12N15/82; C12P5/00; C12P21/00; C12P33/00; C12R1/91; (IPC1-7): C12N15/82; C12N5/10
Attorney, Agent or Firm:
Ryoji Kawase (1 outside)
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