Title:
【発明の名称】トリコテセン-欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法
Document Type and Number:
Japanese Patent JP2002515253
Kind Code:
A
Abstract:
The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the trichodiene synthases. The present invention further relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.
Inventors:
Royer, John Sea.
Christianson, Lean M.
Gambetta, Gregory A.
Brody, Howard
Otani, Suzanne M.
Yoder, Wendy Tea.
Christianson, Lean M.
Gambetta, Gregory A.
Brody, Howard
Otani, Suzanne M.
Yoder, Wendy Tea.
Application Number:
JP2000549743A
Publication Date:
May 28, 2002
Filing Date:
May 20, 1999
Export Citation:
Assignee:
Novozymes Biotech, Incorporated
International Classes:
C12N15/09; C12N1/15; C12N9/02; C12N9/06; C12N9/24; C12N9/88; C12N15/60; C12N15/80; C12P21/02; C12R1/77; (IPC1-7): C12N15/09; C12N1/15; C12N9/24; C12N9/88; C12P21/02
Attorney, Agent or Firm:
Takashi Ishida (4 others)