Title:
【発明の名称】細胞培養培地への金属依存性プロテアーゼ又はキモトリプシンの阻害物質の添加を含む組換えポリペプチドの製造法
Document Type and Number:
Japanese Patent JP2000509994
Kind Code:
A
Abstract:
A process is presented for reducing the detrimental influence of certain proteases on recombinant human protein and polypeptide production in a cell culture, which comprises adding an inhibitor of metal-dependent proteases or chymotrypsins to the cell culture medium. The cell culture medium for cultivating cells expressing and secreting a biologically active recombinant human polypeptide contains an inhibitor of metal-dependent proteases or chymotrypsins, or a combination thereof. Recombinant factor VIII which has been produced in a cell culture medium according to the present process is useful for the manufacture of a medicament for administration to a patient having the symptoms of hemophilia A and for treatment of hemophilia A by administration of a therapeutically effective amount of recombinant factor VIII.
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Inventors:
Adamson, Lacille
Balm, Eritsk
Dixcelius, Johann
Reema Lee, Christina
Balm, Eritsk
Dixcelius, Johann
Reema Lee, Christina
Application Number:
JP54080097A
Publication Date:
August 08, 2000
Filing Date:
May 13, 1997
Export Citation:
Assignee:
Pharmacia and Uppurgeon Arbe
International Classes:
C12N15/09; A61K31/00; A61K38/43; A61P7/00; A61P7/04; C07K14/755; C12N5/10; C12N9/50; C12N15/67; C12P21/02; A61K38/00; (IPC1-7): C12N15/09; A61P7/04; A61K38/43; C07K14/755; C12N5/10; C12N9/50; C12P21/02
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)