【発明の名称】金属キレ―トによる標識を使用する生物学的物質の定量方法

Title:

【発明の名称】金属キレ―トによる標識を使用する生物学的物質の定量方法

Document Type and Number:

Japanese Patent JP2525439

Kind Code:

B2

Abstract:

PCT No. PCT/GB87/00663 Sec. 371 Date Apr. 13, 1989 Sec. 102(e) Date Apr. 13, 1989 PCT Filed Sep. 22, 1987 PCT Pub. No. WO88/02489 PCT Pub. Date Apr. 7, 1988.A biological substance is determined with the use of a marker comprising a lanthanide or other metal ion coupled to the biological substance by means of chelate-forming compound. The chelate complex is dissociated by reduction of the ambient pH to a sufficiently low level to liberate the metal ion and the metal ion is then chelated with a separate chelating chromophore with an increase in the pH to a value closer to the optimum for determination of the metal-chelated chromophore by fluorescence spectroscopy, luminometry, colourometry or the like.

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Inventors:

Dakub, salif

Application Number:

JP50571087A

Publication Date:

August 21, 1996

Filing Date:

September 22, 1987

Export Citation:

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Assignee:

Ekins, Roger Philip
Dakub, salif

International Classes:

C12Q1/68; G01N21/78; G01N33/533; G01N33/536; G01N33/58; (IPC1-7): G01N33/533; C12Q1/68; G01N21/78; G01N33/536; G01N33/58

Domestic Patent References:

JP5968673A
JP61186856A

Other References:

【文献】国際公開86/01604(WO,A)

Attorney, Agent or Firm:

Akira Asamura (2 outside)

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