Title:
【発明の名称】グラム陽性宿主生物を用いてグラム陰性菌の外膜たん白質を分泌する方法
Document Type and Number:
Japanese Patent JPH09508266
Kind Code:
A
Abstract:
PCT No. PCT/DE94/01394 Sec. 371 Date Jul. 18, 1996 Sec. 102(e) Date Jul. 18, 1996 PCT Filed Nov. 21, 1994 PCT Pub. No. WO95/20048 PCT Pub. Date Jul. 27, 1995The invention relates to a process for producing outer membrane proteins of gram-negative bacteria by gram-positive host cells. To this end, a gene structure containing a gene coded for an outer membrane protein is constructed; in front of this gene there is a pro-gene sequence which codes for a propeptide of an export protein of a gram-positive bacterium. In front of the pro-gene sequence there is a pre-gene sequence which codes for a signal peptide of an export protein of a gram-positive bacterium. After the cloning of a gene structure thus constructed into a vector and transformation into a gram-positive bacterium, the outer membrane protein is expressed with a signal peptide and propeptide, transported through the membrane of the gram-positive host cell and released into the test of the culture.
Inventors:
Mens Jöchen
Klose Michael
Zam Hermann
Freuter Roland
Klose Michael
Zam Hermann
Freuter Roland
Application Number:
JP51928595A
Publication Date:
August 26, 1997
Filing Date:
November 21, 1994
Export Citation:
Assignee:
Forschungszentrum Jürich Gesellschaft Mito Beschlenktel Haftung
International Classes:
A61K35/74; A61K38/00; A61K39/02; A61P31/04; C07H21/04; C07K14/245; C07K14/31; C12N1/21; C12N9/20; C12N15/09; C12N15/31; C12N15/62; C12P21/02; G01N33/569; (IPC1-7): C12N15/09; A61K35/74; A61K39/02; C07H21/04; C07K14/31; C12N1/21; C12P21/02; G01N33/569
Attorney, Agent or Firm:
Mitsuyoshi Esaki (3 outside)