Title:
【発明の名称】試料中のオリゴヌクレオチドの検出のための方法およびシステム
Document Type and Number:
Japanese Patent JP2002531100
Kind Code:
A
Abstract:
The present invention provides a method and a system for the detection of a target oligonucleotide in a sample. The method comprises, in general, the steps of providing a sensor device having a sensing interface carrying capturing oligonucleotides having each a nucleotide sequence complementary in at least a portion thereof to a first portion of the target oligonucleotide; providing verification oligonucleotides having each a nucleotide sequence complementary in at least a portion thereof to a second portion of the target oligonucleotide, other than the first portion; contacting the sample with the sensing interface under conditions so as to allow the target oligonucleotides, if present in the sample, to hybridize to the capturing oligonucleotides; prior to contacting the sample with the sensing interface or thereafter, allowing the verification oligonucleotides to hybridize to the target oligonucleotides if present in the sample; and detecting the presence of the verification oligonucleotides on the sensing interface. The detection of the verification oligonucleotides being indicative to the presence of the target nucleotide in the sample.
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Inventors:
Wilner, Itamar
Bardia, Amos
Patrsky, Fernando
Katz, Evgeny
Dagan, Arrier
Bardia, Amos
Patrsky, Fernando
Katz, Evgeny
Dagan, Arrier
Application Number:
JP2000585444A
Publication Date:
September 24, 2002
Filing Date:
December 01, 1999
Export Citation:
Assignee:
Issum Research Development Cumpany of the Hebrew University of Jerusalem
International Classes:
C12N15/09; C12Q1/68; G01N5/02; G01N27/02; G01N27/22; G01N27/416; G01N33/566; G01N33/53; (IPC1-7): C12Q1/68; C12N15/09; G01N5/02; G01N27/02; G01N27/22; G01N27/416; G01N33/53; G01N33/566
Attorney, Agent or Firm:
Sota Asahina (3 outside)