Title:
【発明の名称】タンパク質-多糖コンジュゲートおよびワクチンの製造中に遊離タンパク質を除去するための、接近が制限された媒介材料を用いる簡素化された方法
Document Type and Number:
Japanese Patent JP2002502830
Kind Code:
A
Abstract:
Removing free protein from a liquid mixture containing the protein and a protein-polysaccharide conjugate can be a technologically difficult, expensive, and time consuming process. The procedure described herein for removing the protein from such a mixture simplifies this removal. The procedure includes contacting the liquid mixture, including the protein and the protein-polysaccharide conjugate, with a solid phase, restricted-access media material. This material at least partially binds with the protein and separates it from the liquid mixture, thereby providing a purified liquid containing at least a portion of the protein-polysaccharide conjugate and a reduced amount of the protein. The purified liquid can be collected for further processing or use. As one example, the restricted-access media material can include porous silica particles that generally allow the protein to enter, but restrict or limit access to the protein-polysaccharide conjugate. In this manner, at least some of the free protein adheres, attaches, or otherwise binds to the restricted-access media material and separates from the conjugate. The process of the invention can be used for preparing protein-polysaccharide conjugates that can be used in vaccines or other pharmaceutical compositions.
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Inventors:
Wreath, Andrew
Schaffer, Douglas, E.
Schaffer, Douglas, E.
Application Number:
JP2000530236A
Publication Date:
January 29, 2002
Filing Date:
February 04, 1999
Export Citation:
Assignee:
Henry M. Jackson Foundation for the Advancement of Military Medicine
International Classes:
A61K39/00; A61K39/385; A61K47/48; A61P37/04; B01D15/00; B01D15/08; B01J20/10; B01J20/291; C07K1/20; C12S3/14; (IPC1-7): C07K1/20; A61K39/00; A61K39/385; A61K47/48; B01D15/08
Attorney, Agent or Firm:
Yusuke Hiraki (1 person outside)