Title:
【発明の名称】共有結合された生体高分子を含む親水性の外層によって被覆された固体表面、そのような表面を製造する方法、およびそのための共役体
Document Type and Number:
Japanese Patent JPH06502156
Kind Code:
A
Abstract:
A solid surface having anionic groups capable of reacting with imino groups is provided with a hydrophilic outer surface layer comprising a covalently bonded biopolymer by a method selected from the group consisting of: a) reacting a water-soluble conjugate comprising nonionic hydrophilic polymer covalently bonded to a polyethylene imine and at least partly to a biopolymer with anionic groups on the surface; b) reacting a polyethylene imine substituted by nonionic hydrophilic polymer with anionic groups on the surface, and reacting biopolymer with reactive groups of the nonionic hydrophilic polymer in the presence of a reaction medium having a dielectric constant less than 10% of the dielectric constant of pure water; and c) reacting a polyethylene imine substituted by an anionic hydrophilic polymer derived from a nonionic hydrophilic polymer having a cloud point which is at least 5 DEG C. above the temperature at which the final product is to be used, and having biopolymer-reactive groups, and reacting biopolymer forming covalent bonds with reactive groups of the nonionic polymer in a water-base reaction medium at a temperature which is more than 5 DEG C. below the cloud point of the nonionic hydrophilic polymer in the reaction medium. The product comprises a biopolymer immobilized on a hydrophilic solid surface having a hydrophilic layer, coupled thereto via a polyethylene imine, and accordingly has low spontaneous adsorption of proteins and other biopolymers through electrostatic attraction and/or hydrophobic interaction.
Inventors:
Bergstrom, Karin
Holmberg, Christel
Holmberg, Christel
Application Number:
JP51716691A
Publication Date:
March 10, 1994
Filing Date:
October 18, 1991
Export Citation:
Assignee:
BEROL NOBEL AB
International Classes:
C07K1/113; C07K14/76; C07K17/06; C07K17/08; C07K17/14; C08J7/04; C12N11/06; A61K47/48; C12N11/08; G01N33/543; G01N33/545; (IPC1-7): C07K17/06; A61K47/48; C07K17/14; C08J7/04; C12N11/06; C12N11/08
Attorney, Agent or Firm:
Fukami Hisaro (3 outside)