Title:
【発明の名称】時間分解蛍光測定を使用する蛍光分子群識別のためのシステム
Document Type and Number:
Japanese Patent JP2000500874
Kind Code:
A
Abstract:
PCT No. PCT/EP97/04665 Sec. 371 Date May 22, 1998 Sec. 102(e) Date May 22, 1998 PCT Filed Aug. 27, 1997 PCT Pub. No. WO98/09154 PCT Pub. Date Mar. 5, 1998System and method for distinguishing at least two types of molecule groups that are bound to analyte molecules and which have different fluorescent characteristics. The system performs differentiation using time-resolved fluorescence measurement and includes a light source that exposes a first sample volume to light that is suitable for exciting the at least two types of molecule groups to fluorescence. The system also includes a detector for detecting fluorescence radiation emitted from a second sample volume that at least partially overlaps the first sample volume, and a control unit that is designed to activate the light source for a time interval T1 and, after time interval T2 has passed, to activate the detector for a time interval T3. According to this system, the illumination and detection of emitted fluorescence radiation is performed at least 1,000 times per millisecond, the detector signals recorded with a recording unit during time interval T3 are evaluated using an evaluation unit, and the signal over time during time interval T3 is used to determine which of the at least two groups of molecules is contained in the overlapping sample volume.
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Inventors:
Muller, Ralph
Sauer, Marx
Tsunder, Christoph
Sauer, Marx
Tsunder, Christoph
Application Number:
JP51128498A
Publication Date:
January 25, 2000
Filing Date:
August 27, 1997
Export Citation:
Assignee:
Roche Diagnostic Gesell Shaft Mitt Beschlenktel Haftung
International Classes:
C12Q1/68; C12Q1/6816; G01N21/64; G01N27/447; G01N33/543; (IPC1-7): G01N21/64; G01N27/447; G01N33/543
Domestic Patent References:
| JPH05126739A | 1993-05-21 | |||
| JPH06148076A | 1994-05-27 | |||
| JPH02234050A | 1990-09-17 | |||
| JPH01227948A | 1989-09-12 | |||
| JPS6435268A | 1989-02-06 | |||
| JPH07505467A | 1995-06-15 |
Attorney, Agent or Firm:
Sota Asahina (1 person outside)