Title:
【発明の名称】哺乳動物細胞における遺伝子発現
Document Type and Number:
Japanese Patent JPH11502705
Kind Code:
A
Abstract:
Proteins such as human beta-interferon or human erythropoietin are prepared by culturing mammalian cells which harbour a nucleic acid sequence comprising: (i) a coding sequence which encodes the desired protein and which is operably linked to a promoter capable of directing expression of the coding sequence in a mammalian cell in the presence of a heavy metal ion; and (ii) a first selectable marker sequence comprises a metallothionein gene and which is operably linked to a promoter capable of directing expression of the metallothionein gene in a mammalian cell in the presence of a heavy metal ion; and optionally (iii) a second selectable marker sequence which comprises a neo gene and which is operably linked to a promoter capable of directing expression of the neo gene in a mammalian cell.
Inventors:
Tan, In Wee
Hong, Wang Jin
Hong, Wang Jin
Application Number:
JP52873096A
Publication Date:
March 09, 1999
Filing Date:
March 22, 1996
Export Citation:
Assignee:
Tan, In Wee
Hong, Wang Jin
Hong, Wang Jin
International Classes:
A61K38/21; A61K38/22; C07K14/505; C07K14/565; C07K14/825; C12N5/10; C12N15/22; C12N15/85; C12P21/02; C12N15/09; A61K38/00; C12R1/91; (IPC1-7): C12N15/09; A61K38/21; A61K38/22; C07K14/505; C07K14/565; C07K14/825; C12N5/10; C12P21/02
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)