Title:
【発明の名称】膵臓幹細胞および移植におけるその使用
Document Type and Number:
Japanese Patent JP2003523323
Kind Code:
A
Abstract:
Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin has been identified as a molecular marker for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.
Inventors:
Abraham, Elizabeth, Jay.
Faust man, dennis
Havenor, Joel, F.
Mario, Valeggio
Trevski, Henry
Thomas, Melissa, Kay.
Faust man, dennis
Havenor, Joel, F.
Mario, Valeggio
Trevski, Henry
Thomas, Melissa, Kay.
Application Number:
JP2001541516A
Publication Date:
August 05, 2003
Filing Date:
December 06, 2000
Export Citation:
Assignee:
THE GENERAL HOSPITAL CORPORATION
International Classes:
A01K67/027; A61K31/436; A61K31/7088; A61K35/39; A61K38/00; A61K38/22; A61K39/395; A61K45/00; C12N15/09; A61K48/00; A61P3/10; A61P37/06; A61P43/00; C12N5/02; C12N5/071; C12N5/074; C12Q1/00; G01N33/53; A61K35/12; A61K; C12N; (IPC1-7): A61K35/12; A61K31/436; A61K38/00; A61K39/395; A61K45/00; A61P3/10; A61P37/06; A61P43/00; C12N5/06
Foreign References:
| WO1997015310A1 | 1997-05-01 | |||
| WO1997015310A1 | 1997-05-01 |
Other References:
JPN5002022447, ＣＯＲＮＥＬＩＵＳ Ｊ Ｇ, ＨＯＲＭ ＭＥＴ ＲＥＳ, 1997, Ｖ２９ Ｎ６, Ｐ２７１−２７７
JPN6010051054, Ｐｒｏｃ． Ｎａｔｌ． Ａｃａｄ． Ｓｃｉ． ＵＳＡ, 1987, ｖｏｌ． ８４, ６５５５−６５５７
JPN6010051054, Ｐｒｏｃ． Ｎａｔｌ． Ａｃａｄ． Ｓｃｉ． ＵＳＡ, 1987, ｖｏｌ． ８４, ６５５５−６５５７
Attorney, Agent or Firm:
Yoshinori Hosoda