To obtain an immobilized monomethine cyanine dye which is represented by a specific formula, emits light in the near-UV and blue regions, has desired spectral characteristics and is useful as a fluorescent marker in a variety of bilogical and non-biological purposes.
This compound is represent by an arbitrarily R2-R7-substituted formula I (R1-R2 are each selected so that this compound are imparted desired solubility, reactivity and spectral characteristics; T is a bonding group having a formula II of a 6 or 7-membered ring; X and Y are each a bis-substituted C, O, S, Se or CH=CH; Z1 and Z2 each represent an atom necessary to complete one, two or three condensed rings and are selected from C, O, N and S atoms), typically 6,6-disulfo-meso-carboxymethyl bis-(benzothiazolyl)methineboronfluoride. This cyanine dye is prepared by reaction of a compound of formula III with a compound suitable for formation of the bond, T.
ARAN ESU WAGOONAA
BARUCHIYANDORA EMU KARANDEIKAR
