Title:
免疫反応測定方法およびそれに用いる免疫反応測定用試薬
Document Type and Number:
Japanese Patent JP4512492
Kind Code:
B2
Abstract:
The present invention relates to an immunoreaction measuring method for measuring an antigen or antibody contained as a subject substance in a sample, wherein the sample was mixed with at least one compound selected from the group consisting of dicarboxylic acids having a hydroxyl group, dicarboxylic acids having a double bond, straight-chain dicarboxylic acids expressed by the chemical formula (1): HOOC(CH2)nCOOH (n is an integer), and the salts of these dicarboxylic acids, and an antibody or antigen as a specifically binding substance capable of specifically binding to the subject substance, to obtain an acidic reaction solution, and then an antigen-antibody complex, generated by an antigen-antibody reaction of the subject substance with the specifically binding substance in the reaction solution was detected. This makes it possible to improve a measurement value and to relax a limitation of a measurement range due to a zone phenomenon that occurs in an antigen-excess region.
Inventors:
Akihito Kamei
Tatsuro Kawamura
Yukawa Keiko
Tatsuro Kawamura
Yukawa Keiko
Application Number:
JP2004558449A
Publication Date:
July 28, 2010
Filing Date:
December 09, 2003
Export Citation:
Assignee:
Panasonic Corporation
International Classes:
G01N33/531; G01N33/53; G01N33/543
Domestic Patent References:
| JPH0682450A | 1994-03-22 |
Attorney, Agent or Firm:
Kazuo Ishii
Shinichi Kawasaki
Koichi Naka
Shinichi Kawasaki
Koichi Naka