Title:
IMMUNOCONJUGATES AND PREPARATION METHODS THEREOF 2
Document Type and Number:
Japanese Patent JP2017122084
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a protein comprising an immunoglobulin variable region modified for promoting conjugation of a compound to the protein or such a protein with a compound conjugated thereto.SOLUTION: Provided is an isolated protein comprising an immunoglobulin variable region that possesses at least two cysteine residues positioned in the framework region (FR)2 and/or at least two cysteine residues positioned in the framework region (FR)3, where if at least two of the cysteine residues in FR2 and/or FR3 are not conjugated to a compound, a disulfide bond in the framework can be formed between the cysteine residues. Preferably, the protein comprises an immunoglobulin heavy chain variable region (V) and an immunoglobulin light chain variable region (V), and at least one of the variable regions contains two cysteine residues. Also the protein may be conjugated to other compounds.SELECTED DRAWING: None
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Inventors:
HUDSON PETER JOHN
DEBRA TAMVAKIS
MICHAEL PAUL WHEATCROFT
FABIO TURATTI
DEBRA TAMVAKIS
MICHAEL PAUL WHEATCROFT
FABIO TURATTI
Application Number:
JP2017000559A
Publication Date:
July 13, 2017
Filing Date:
January 05, 2017
Export Citation:
Assignee:
AVIPEP PTY LTD
International Classes:
C07K16/18; A61K38/00; A61K47/50; A61P35/00; A61P35/02; A61P37/02; A61P37/06; C07K16/22; C07K16/28; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; G01N33/53
Domestic Patent References:
| JP2008506659A | 2008-03-06 | |||
| JP2008516896A | 2008-05-22 |
Foreign References:
| WO2003050531A2 | 2003-06-19 |
Other References:
NAT. BIOTECHNOL., vol. 26, no. 8, JPN6014036821, 2008, pages 925 - 932, ISSN: 0003732349
J. IMMUNOL. METHODS, vol. vol.332, no.1-2, JPN6014036819, 2008, pages 41 - 52, ISSN: 0003732350
BIOCONJUGATE CHEMISTRY, vol. 19, JPN6015052937, 2008, pages 2527 - 2534, ISSN: 0003732351
VOYNOV VLADIMIR: "DESIGN AND APPLICATION OF ANTIBODY CYSTEINE VARIANTS", BIOCONJUGATE CHEMISTRY, vol. V21 N2, JPN5012014265, 17 February 2010 (2010-02-17), pages 385 - 392, ISSN: 0003732352
SAERENS ET AL., J. MOL. BIOL., vol. 377, JPN6015007147, pages 478 - 488, ISSN: 0003732353
WEBBER ET AL., MOLECULAR IMMUNOLOGY, vol. 32, no. 4, JPN6015007149, pages 249 - 258, ISSN: 0003732354
J. IMMUNOL. METHODS, vol. vol.332, no.1-2, JPN6014036819, 2008, pages 41 - 52, ISSN: 0003732350
BIOCONJUGATE CHEMISTRY, vol. 19, JPN6015052937, 2008, pages 2527 - 2534, ISSN: 0003732351
VOYNOV VLADIMIR: "DESIGN AND APPLICATION OF ANTIBODY CYSTEINE VARIANTS", BIOCONJUGATE CHEMISTRY, vol. V21 N2, JPN5012014265, 17 February 2010 (2010-02-17), pages 385 - 392, ISSN: 0003732352
SAERENS ET AL., J. MOL. BIOL., vol. 377, JPN6015007147, pages 478 - 488, ISSN: 0003732353
WEBBER ET AL., MOLECULAR IMMUNOLOGY, vol. 32, no. 4, JPN6015007149, pages 249 - 258, ISSN: 0003732354
Attorney, Agent or Firm:
Hiroshi Kobayashi
Norio Omori
Yasuhito Suzuki
Norio Omori
Yasuhito Suzuki