Title:
免疫原性の解毒変異体E.coli LT―A毒素
Document Type and Number:
Japanese Patent JP3715326
Kind Code:
B2
Abstract:
An immunogenic detoxified protein is provided which comprises the amino acid sequence of subunit A of an E.coli heat labile toxin (LT-A) or a fragment thereof in which at least amino acid Ala-72 of the A subunit is mutated, preferably by substitution with Arg. The toxoid is useful as vaccine against an enterotoxigenic strain of E.coli and is produced by recombinant DNA means by site-directed mutagenesis. It is also an effective adjuvant.
Inventors:
Pizza, Maria Grazia
Giuliani, Marzia Monica
Lapuoli, Reno
Giuliani, Marzia Monica
Lapuoli, Reno
Application Number:
JP52024098A
Publication Date:
November 09, 2005
Filing Date:
October 30, 1997
Export Citation:
Assignee:
Chiron Sochieta A Responsor Billita Limitata
International Classes:
A01K67/027; A61K39/00; A61K39/108; A61K39/39; A61P1/12; A61P31/04; A61P37/04; C07K14/245; C12N1/21; C12N15/09; C12N15/31; C12Q1/48; A61K38/00; C12R1/19; (IPC1-7): A61K39/108; A61K39/39; A61P37/04
Foreign References:
| WO1993013202A1 | ||||
| WO1995017211A1 |
Other References:
PIZZA M. et al., Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis, Molecular Microbiology,1994年,Vol.14, No.1, 51-60
GIULIANI M.M. et al., Mucosal Adjuvancity and immunogenicity of LTR72, a novel mutant of Escherichia coli heat-labile ente, J. Exp. Med., 米国,1998年 4月 6日,Vol.187, No.7, 1123-1132
GIULIANI M.M. et al., Mucosal Adjuvancity and immunogenicity of LTR72, a novel mutant of Escherichia coli heat-labile ente, J. Exp. Med., 米国,1998年 4月 6日,Vol.187, No.7, 1123-1132
Attorney, Agent or Firm:
Hidesaku Yamamoto