Title:
IMMUNOLOGICAL MEASURING METHOD AND ITS DEVICE
Document Type and Number:
Japanese Patent JP3436982
Kind Code:
B2
Abstract:
PURPOSE: To simply determine an immune substance qualitatively or quantitatively with a fluorescent immuno-assey and/or light emitting immuno-assey through the absorptive measuring of an immune complex body.
CONSTITUTION: At least one of the surfaces of an total reflection cell 2 is formed as an total reflection prisim 4. A mixture liquid 8 containing a gold colloid marker antibody 6 formed by adsorbing antibody to a gold colloid is put in the total reflection cell 2, and thereto a mixture liquid as specimen 12 is added which contains an antigen 10 to make antigen-antibody reaction with the antibody, and thereby a gold colloid marker immune complex 14 is produced. From an incident optical system 16, the measuring light is put incident at an incident angle which generates a total reflection to the total reflection prism 4, and the emissive light therefrom is received by a measuring optical system 18, and thereby absorption by the complex 14 is measured to serve for qualitative and quantitative determination of the antigen 10 contained in the specimen liquid 12.
Inventors:
Dou Akomei
Yoshinori Yamaguchi
Seizo Uenoyama
Yoshinori Yamaguchi
Seizo Uenoyama
Application Number:
JP20274094A
Publication Date:
August 18, 2003
Filing Date:
August 03, 1994
Export Citation:
Assignee:
ARKRAY, Inc.
International Classes:
G01N33/542; B82Y15/00; G01N21/27; G01N21/552; G01N33/536; G01N33/543; G01N33/58; (IPC1-7): G01N33/536; G01N21/27
Domestic Patent References:
JP638560A | ||||
JP4233465A | ||||
JP7146295A | ||||
JP5203574A | ||||
JP6118083A | ||||
JP694719A | ||||
JP372263A | ||||
JP61226644A | ||||
JP5142142A |
Attorney, Agent or Firm:
Shigeo Noguchi
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