Title:
マルチカラーリアルタイムPCR用の改良されたシステム
Document Type and Number:
Japanese Patent JP5144605
Kind Code:
B2
Abstract:
The invention is directed to a system for performing multi-color real time PCR, comprising a flexible real time PCR instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
Inventors:
Zagner, Gregor
Bechler, english
Volte, Joachim
Heindle, Dieter
Josel, Hans-Peter
Gutekunst, Martin
Zybru, Rudolph
Muller, Christoph
Bechler, english
Volte, Joachim
Heindle, Dieter
Josel, Hans-Peter
Gutekunst, Martin
Zybru, Rudolph
Muller, Christoph
Application Number:
JP2009170421A
Publication Date:
February 13, 2013
Filing Date:
July 21, 2009
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12M1/00; C12N15/09; C12Q1/68; G01N21/64
Domestic Patent References:
| JP2002515602A | ||||
| JP2000511435A | ||||
| JP2000512138A | ||||
| JP2000509608A | ||||
| JP8510562A | ||||
| JP6110730A | ||||
| JP62206418A |
Other References:
”Rotor-Gene 3000 Four-Channel Multiplexing System”,(2002),p.1-8
BioTechniques,27(1999),p.342-349
BioTechniques,22(1997),p.176-181
Methods,25(2001),p.430-442
Cem Euro J.,9[7](2003.04.04),p.1479-1485
BioTechniques,27(1999),p.342-349
BioTechniques,22(1997),p.176-181
Methods,25(2001),p.430-442
Cem Euro J.,9[7](2003.04.04),p.1479-1485
Attorney, Agent or Firm:
Yoshinori Hosoda