Title:
ポリマー粒子の改良
Document Type and Number:
Japanese Patent JP2007529487
Kind Code:
A
Abstract:
A process for covalently binding a tagged protein to a polymer particle comprising: contacting a tagged protein with a chelating agent-polymer particle conjugate wherein said tag comprises at least two histidine residues and at least two lysine residues and said chelating agent is tridentate, tetradentate or pentadentate and comprises at least two carboxyl groups and is coordinated by Co2+ ions, to form particle-chelating agent Co2+ complex: contacting said complex with a carbodiimide; and optionally removing the Co2+ ions.
Inventors:
Song Jae, Pal
Application Number:
JP2007503405A
Publication Date:
October 25, 2007
Filing Date:
March 17, 2005
Export Citation:
Assignee:
Invitrogen Dyna AS
International Classes:
C07K1/22; B01J20/26; B01J20/32; B01J45/00; B03C1/00; C07K2/00; C08J3/12; G01N33/543
Domestic Patent References:
| JP2003531213A | 2003-10-21 | |||
| JP2001506968A | 2001-05-29 |
Foreign References:
| US20040029781A1 | 2004-02-12 | |||
| US6441146B1 | 2002-08-27 | |||
| US4654267A | 1987-03-31 |
Attorney, Agent or Firm:
Hatsushi Shimizu
Koichi Niimi
Koichi Niimi