Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
IN-LIQUID GRAM STAINING METHOD AND MICROORGANISM TEST METHOD
Document Type and Number:
Japanese Patent JP2013198410
Kind Code:
A
Abstract:

To provide a gram staining method, a microorganism test method, and an apparatus applicable even when a microorganism concentration is low and capable of recovering microorganisms after carrying out gram staining to allow the execution of an identification and drug sensitivity examination in a post-process.

A gram staining method for microorganisms includes the steps of: treating microorganisms in a series of gram staining solutions by repeating (a) concentration or capture of microorganisms, (b) solvent substitution, and (c) suspension of microorganisms, in a container containing a solution that contains or is suspected to contain microorganisms; and determining gram positive bacteria or gram negative bacteria from the stained condition of microorganisms. Gram staining is performed in a state of holding the form of microorganisms, and microorganism recovery after determination is simplified to permit the execution of an identification and drug sensitivity examination of microorganisms using the gram-stained microorganisms.


Inventors:
KIKUCHI YUKI
UEMATSU CHIHIRO
MAEJIMA MUNEO
SHOJI YASUNORI
MATSUI TAKUYA
Application Number:
JP2012067224A
Publication Date:
October 03, 2013
Filing Date:
March 23, 2012
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
HITACHI HIGH TECH CORP
International Classes:
C12Q1/04; C12M1/34; C12Q1/68; G01N1/10; G01N1/30; G01N33/50
Domestic Patent References:
JP2007121282A2007-05-17
JP2006042743A2006-02-16
JP2009219383A2009-10-01
JP2007252393A2007-10-04
JP2007014753A2007-01-25
JP2010529895A2010-09-02
Other References:
JPN6015030105; Appl. Environ. Microbiol. Vol.64, No.7, 1998, pp.2681-2685
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita