Title:
IN VITRO SKIN MODEL COMPOSITION FOR EVALUATING SKIN STIMULATION AND EVALUATION METHOD USING THE SAME
Document Type and Number:
Japanese Patent JP2013021946
Kind Code:
A
Abstract:
To provide a skin model for evaluating skin stimulation which can be conveniently prepared and stably supplied, and in which quantitative evaluation can be performed.
There are disclosed an in vitro skin model composition for evaluating skin stimulation comprising a planar keratinocyte culture material area, and a nerve cell culture material area in which nerve cells are planarly disseminated so as to adjoin non-contactly and at regular intervals to the border of the planar keratinocyte culture material area, wherein the nerve cells proceed into the keratinocyte culture material area by extending axons thereof, a preparation method thereof, and an evaluation method using the same.
Inventors:
DENDA MITSUHIRO
SHINODA MOE
DENDA SUMIKO
NAKAYA MASASHI
SHINODA MOE
DENDA SUMIKO
NAKAYA MASASHI
Application Number:
JP2011158013A
Publication Date:
February 04, 2013
Filing Date:
July 19, 2011
Export Citation:
Assignee:
SHISEIDO CO LTD
International Classes:
C12N5/071; C12N5/0793; C12Q1/02; G01N33/48; G01N33/483; G01N33/50
Domestic Patent References:
| JP2007515958A | 2007-06-21 |
Foreign References:
| WO2003005023A2 | 2003-01-16 | |||
| WO2005071065A1 | 2005-08-04 |
Other References:
EXPERIMENTAL DERMATOLOGY, vol. 20, JPN6015035676, June 2011 (2011-06-01), pages 464 - 467, ISSN: 0003148293
JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 127, JPN6015035687, 2007, pages 979 - 981, ISSN: 0003148294
NEUROSCIENCE, vol. 159, JPN6015035678, 2009, pages 514 - 525, ISSN: 0003148295
EUROPEAN JOURNAL OF NEUROSCIENCE, vol. 26, JPN6015035682, 2007, pages 113 - 125, ISSN: 0003148296
BIOSENSORS AND BIOELECTRONICS, vol. 21, JPN6015035683, 2006, pages 1093 - 1100, ISSN: 0003148297
JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 127, JPN6015035687, 2007, pages 979 - 981, ISSN: 0003148294
NEUROSCIENCE, vol. 159, JPN6015035678, 2009, pages 514 - 525, ISSN: 0003148295
EUROPEAN JOURNAL OF NEUROSCIENCE, vol. 26, JPN6015035682, 2007, pages 113 - 125, ISSN: 0003148296
BIOSENSORS AND BIOELECTRONICS, vol. 21, JPN6015035683, 2006, pages 1093 - 1100, ISSN: 0003148297
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Masaru Nakajima
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Masaru Nakajima
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