Title:
RNA依存性RNAポリメラーゼ(RDRP)ウイルスの複製のためのインビトロ系
Document Type and Number:
Japanese Patent JP2004537271
Kind Code:
A
Abstract:
An in vitro method to conduct genomic replication of the viral genomes of viruses that utilize RNA-dependent RNA polymerase for replication (RDRP viruses), such as HCV. The method employs a construct comprising the 3' and 5' untranslated regions (UTRs) of the viral genome which are operably linked on the 5' and 3' ends of a reporter sequence, in antisense orientation, such that when viral replication is occurring within the cell which produces RDRP, the reporter protein will be made. The method of the invention provides an efficient means for measuring genomic replication in RDRP viruses, and also for the rapid screening of compounds for their ability to inhibit genomic replication of RDRP viruses, including the Hepatitis C virus (HCV).
Inventors:
Robert W King
Matthew W. Jeffreys
Claudio Paskinelli
Matthew W. Jeffreys
Claudio Paskinelli
Application Number:
JP2002561605A
Publication Date:
December 16, 2004
Filing Date:
January 31, 2002
Export Citation:
Assignee:
BRISTOL-MYERS SQUIBB PHARMA COMPANY
International Classes:
A61K39/29; C12N5/00; C12N5/02; C12N5/10; C12N7/00; C12N15/09; C12N7/01; C12N15/40; C12N15/51; C12N15/85; C12P19/34; C12Q1/02; C12Q1/20; C12Q1/25; C12Q1/66; C12Q1/70; G01N33/53; G01N33/537; G01N33/543; (IPC1-7): C12N15/09; C12N5/10; C12Q1/02; C12Q1/66
Attorney, Agent or Firm:
Mitsuo Tanaka
Yushiro Tsuboi
Nagatoshi Shinagawa
Yushiro Tsuboi
Nagatoshi Shinagawa