PURPOSE: To treat a disease caused by the elevation of TNF-α concentration in the blood by preparing a therapeutic agent containing a F(ab')2-and/or a F(ab)fragment of a monoclonal antibody mAb195 as active ingredients.
CONSTITUTION: This method for preparing a fragment of a monoclonal antibody comprises cleaving a monoclonal antibody mAb195 by a pepsin at pH 4.5 in the presence of 10.5 M NaCl, removing the separated Fc fragment by a centrifugal separation, then treating a supernatant with a chromatogram on a Sephacryl (trade mark of Pharmacia) S-200 gel filtration column, then filtering the combined fractions containing a F(ab')2-fragment by using a Protein-A-sepharose (trade mark of Pharmacia) for producing the F(ab')2 fragment. This fragment can be used for the treatment of a disease in which the TNF-α concentration in the blood is elevated, e.g. septic shock.
FURANTSU EMURINGU
MANFUREETO KURUFUYURUSUTO
TOOMASU MEIYAA
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