INACTIVATED NUCLEIC ACID PROBE AND SUPPORTER FOR SOLID BODY OF NUCLEIC ACIDS

Title:

INACTIVATED NUCLEIC ACID PROBE AND SUPPORTER FOR SOLID BODY OF NUCLEIC ACIDS

Document Type and Number:

Japanese Patent JPS6036496

Kind Code:

A

Abstract:

A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive intercalator compound or other nucleic acid-binding ligands, and (c) divalent radical chemically linking the substrate and the ligand (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanediol-diglycidyl ether to an amino-substituted angelicin or psoralen or ethidium bromide which in turn is photochemically linked to a nucleic acid. The resulting immobilized nucleic acid probe is capable of hybridizing with complementary nucleic acid fragments and is thereby useful in diagnostic assays.

Inventors:

NANIBUFUSHIYAN DATSUTAGUPUTA
DONARUDO EMU KUROZAAZU

Application Number:

JP13804684A

Publication Date:

February 25, 1985

Filing Date:

July 05, 1984

Export Citation:

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Assignee:

MOREKIYURAA DAIAGUNOSUTEITSUKU

International Classes:

C07H21/00; C07H21/04; C09H1/00; C08H1/00; C12Q1/68; G01N33/53; G01N33/547; G01N33/548; (IPC1-7): C07H21/00; C08H1/00

Attorney, Agent or Firm:

Heikichi Odashima

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