Title:
ヒト多能性幹細胞から視床下部ニューロンへの分化誘導
Document Type and Number:
Japanese Patent JP7023496
Kind Code:
B2
Abstract:
There is provided a method for efficient differentiation induction from human pluripotent stem cells into hypothalamic neurons. Also, provided is a method for constructing, from human pluripotent stem cells, a cellular structure in which hypothalamic tissue and pituitary tissue are integrated. A cellular structure including hypothalamic tissue is obtained by a method including the steps of: culturing an aggregate of human pluripotent stem cells in suspension in a medium containing a low concentration of a bone morphogenetic protein signal transduction pathway activating substance and a low concentration of a substance acting on the Shh signaling pathway; and further culturing the cell aggregate obtained in the step in suspension in a medium containing a low concentration of a substance acting on the Shh signaling pathway. Also, a cellular structure including hypothalamic tissue and pituitary tissue is obtained by a method including the steps of: culturing an aggregate of human pluripotent stem cells in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating substance and a substance acting on the Shh signaling pathway; further culturing the cell aggregate formed in the step in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating substance and a substance acting on the Shh signaling pathway; and culturing the cell aggregate obtained in the step in suspension in a medium suitable for simultaneous induction of pituitary and hypothalamus.
Inventors:
Hidetaka Suga
Koichiro Ogawa
Takatoshi Kasai
Hiro Arima
Koichiro Ogawa
Takatoshi Kasai
Hiro Arima
Application Number:
JP2017562850A
Publication Date:
February 22, 2022
Filing Date:
January 18, 2017
Export Citation:
Assignee:
Tokai National University Organization
International Classes:
C12N5/079; C12N5/0735; C12N5/10
Foreign References:
| WO2015076388A1 | ||||
| WO2014153230A1 | ||||
| WO2013065763A1 |
Other References:
ブレインサイエンス・レビュー, 2014, Vol.2014, pp.99-112
Nat. Commun., 2016.01.14, 7:10351
Development, 2015, Vol.142, pp.633-643
Endocrine Journal, 2010, Vol.57, No.1, pp.3-14
Proc. Natl. Acad. Sci. USA, 2008, Vol.105, No.33, pp.11796-11801
現代医学, 2013, Vol.61, No.2, pp.191-199
Dev. Biol., 2010, Vol.348, No.2, pp.199-209
Stem Cell Research, 2015, Vol.15, pp.290-298
Nat. Commun., 2016.01.14, 7:10351
Development, 2015, Vol.142, pp.633-643
Endocrine Journal, 2010, Vol.57, No.1, pp.3-14
Proc. Natl. Acad. Sci. USA, 2008, Vol.105, No.33, pp.11796-11801
現代医学, 2013, Vol.61, No.2, pp.191-199
Dev. Biol., 2010, Vol.348, No.2, pp.199-209
Stem Cell Research, 2015, Vol.15, pp.290-298
Attorney, Agent or Firm:
Patent Business Corporation Saegusa International Patent Office