Title:
α７受容体結合コリン作動性アゴニストを用いる炎症の阻害
Document Type and Number:
Japanese Patent JP5925178
Kind Code:
B2
Abstract:
Methods of inhibiting release of a proinflammatory cytokine from a macrophage are provided. The methods comprise treating the macrophage with a cholinergic agonist in an amount sufficient to decrease the amount of the proinflammatory cytokine that is released from the macrophage, wherein the cholinergic agonist is selective for an ±7 nicotinic receptor. Methods for inhibiting an inflammatory cytokine cascade in a patient are also provided. The methods comprise treating the patient with a cholinergic agonist in an amount sufficient to inhibit the inflammatory cytokine cascade, wherein the cholinergic agonist is selective for an ±7 nicotinic receptor. Methods for determining whether a compound is a cholinergic agonist reactive with an ±7 nicotinic receptor are also provided. The methods comprise determining whether the compound inhibits release of a proinflammatory cytokine from a mammalian cell. Additionally, methods for determining whether a compound is a cholinergic antagonist reactive with an ±7 nicotinic receptor are provided. These methods comprise determining whether the compound reduces the ability of a cholinergic agonist to inhibit the release of a proinflammatory cytokine from a mammalian cell. Oligonucleotides or mimetics capable of inhibiting attenuation of lipopolysaccharide-induced TNF release from a mammalian macrophage upon exposure of the macrophage to a cholinergic agonist are also provided. The oligonucleotides or mimetics consist essentially of a sequence greater than 5 nucleotides long that is complementary to an mRNA of an ±7 receptor. Additionally, methods of inhibiting attenuation of TNF release from a mammalian macrophage upon exposure of the macrophage to a cholinergic agonist are provided. These methods comprise treating the macrophage with the above-described oligonucleotide or mimetic.
Inventors:
Tracy, Kevin, Jay.
One, Hong
One, Hong
Application Number:
JP2013257181A
Publication Date:
May 25, 2016
Filing Date:
December 12, 2013
Export Citation:
Assignee:
The Finestein Institute for Medical Research
International Classes:
A61K31/444; A61K31/00; A61K31/439; A61K31/46; A61P1/00; A61P1/04; A61P9/00; A61P11/00; A61P15/00; A61P29/00; A61P31/00; A61P33/00; A61P37/00; A61P43/00; G01N33/68; G01N33/94
Foreign References:
| WO2002030405A2 | ||||
| WO1999010338A2 |
Attorney, Agent or Firm:
Yoshinori Hosoda