Title:
INSPECTION METHOD
Document Type and Number:
Japanese Patent JP2015072226
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide an inspection method in which antigens for skin diagnosis contained in a specimen can be rapidly detected with high sensitivity by utilizing antigen-antibody reaction.SOLUTION: Provided is a detection method for detecting particular antigens 51 for skin diagnosis contained in a specimen 50. The detection method includes: a first step for preparing a detection container 1 which is provided with a substrate having a plurality of holes 11 for retaining the specimen 50, specific antibodies 41 for specifically recognizing the antigens 51, and polymers 31 having a first unit containing a phosphorylcholine group and a second unit containing a carboxylic acid derivative group, and in which the specific antibodies 41 are carried on a bottom surface (an inner surface) 21 of the holes 11 via the polymers 31, and for preparing the specimen 50 containing the antigens 51, a surfactant, bicine, and a salt; a second step for supplying the specimen 50 to the holes 11 to let the specific antibodies 41 recognize the antigens 51; and a third step for observing the antigens 51 recognized by the specific antibodies 41.
Inventors:
FUNAOKA SOHEI
YAMANOUCHI GO
YASUDA TOMONAGA
ISHIWATARI SHIOJI
MATSUKUMA SACHIKO
YAMANOUCHI GO
YASUDA TOMONAGA
ISHIWATARI SHIOJI
MATSUKUMA SACHIKO
Application Number:
JP2013208643A
Publication Date:
April 16, 2015
Filing Date:
October 03, 2013
Export Citation:
Assignee:
SUMITOMO BAKELITE CO
FANCL CORP
FANCL CORP
International Classes:
G01N33/53; C12Q1/37; G01N33/50; G01N33/543; G01N33/573
Domestic Patent References:
| JP2013527169A | 2013-06-27 | |||
| JP2007285835A | 2007-11-01 | |||
| JP2012189581A | 2012-10-04 | |||
| JP2009112255A | 2009-05-28 | |||
| JP2002526060A | 2002-08-20 | |||
| JPH10221340A | 1998-08-21 |
Foreign References:
| WO2007046463A1 | 2007-04-26 |
Attorney, Agent or Firm:
Tatsuya Masuda
Kazuo Asahi
Kazuo Asahi