Title:
細胞内におけるＤＮＡ増幅方法
Document Type and Number:
Japanese Patent JP5717443
Kind Code:
B2
Abstract:
The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii):
(i) DNA encoding a protein selected from the following 1), 2) and 3):
1) a protein consisting of the amino acid sequence of SEQ ID NO: 1,
2) a protein comprising an amino acid sequence which has a deletion, substitution, insertion or addition of one or more amino acids in the amino acid sequence of SEQ ID NO: 1, and
3) a protein comprising an amino acid sequence which has an identity of 90% or more to the amino acid sequence of SEQ ID NO: 1,
(ii) DNA consisting of the nucleotide sequence of SEQ ID NO: 2, and
(iii) DNA hybridizing to the nucleotide sequence of SEQ ID NO: 2 under stringent conditions.
Inventors:
Takeshi Murakami
Naomi Sumita
Yanai Koji
Naomi Sumita
Yanai Koji
Application Number:
JP2010515933A
Publication Date:
May 13, 2015
Filing Date:
June 05, 2009
Export Citation:
Assignee:
meiji seika Pharma Co., Ltd.
International Classes:
C12N15/09; C07K14/36; C12N1/21
Foreign References:
| US5240858A | 1993-08-31 |
Other References:
JPN6009031822; バイオサイエンスとインダストリー Ｖｏｌ． ６５， Ｎｏ． ５, 2007, ｐｐ．２３２-２３６
JPN6009031823; Ｐｒｏｃ． Ｎａｔｌ． Ａｃａｄ． Ｓｃｉ． Ｕ．Ｓ．Ａ． Ｖｏｌ． １０３， Ｎｏ． ２５, 2006, ｐｐ．９６６１-９６６６
JPN6009031822; バイオサイエンスとインダストリー Ｖｏｌ． ６５， Ｎｏ． ５, 2007, ｐｐ．２３２-２３６
JPN6009031823; Ｐｒｏｃ． Ｎａｔｌ． Ａｃａｄ． Ｓｃｉ． Ｕ．Ｓ．Ａ． Ｖｏｌ． １０３， Ｎｏ． ２５, 2006, ｐｐ．９６６１-９６６６
JPN6009031822; バイオサイエンスとインダストリー Ｖｏｌ． ６５， Ｎｏ． ５, 2007, ｐｐ．２３２-２３６
Attorney, Agent or Firm:
Hirohito Katsunuma
Hiroyuki Fujii
Hiroshi Sorimachi
Hiroyuki Fujii
Hiroshi Sorimachi